Enzymes that keep DNA under control
نویسندگان
چکیده
منابع مشابه
How bacterial cells keep ribonucleases under control.
Ribonucleases (RNases) play an essential role in essentially every aspect of RNA metabolism, but they also can be destructive enzymes that need to be regulated to avoid unwanted degradation of RNA molecules. As a consequence, cells have evolved multiple strategies to protect RNAs against RNase action. They also utilize a variety of mechanisms to regulate the RNases themselves. These include pos...
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N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated fusion enzymes that preferentially nick 5mCG-modified DNA. The CG dinucleotide can be modified b...
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3T3 cells were established after a temporary dip in reproductive ability (top); once established they entered a resting state at a low cell density (bottom). G RE EN George Todaro and Howard Green establish the 3T3 cell line— the first well behaved, contact-inhibited cell line. In this mess of uncontrolled growth it was difficult or impossible to pick out cells that had been transformed after i...
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ژورنال
عنوان ژورنال: EMBO reports
سال: 2001
ISSN: 1469-221X,1469-3178
DOI: 10.1093/embo-reports/kve073